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RNA-Seq Library Construction Kit

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Kód produktu: NGRR-100

MCLAB's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed cDNA libraries for high-throughput sequencing.

Více informací

Description:
MCLAB's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed cDNA libraries for high-throughput sequencing. This kit can be used to convert RNA transcripts into cDNA whole transcriptome libraries or small RNA libraries for next-generation sequencing analysis using Illumina^® GAIIx^TM, HiSeq^TM 2000 and MiSeq^® sequencing platforms. With preserving the complexity of sequencing libraries, the protocol is quicker than the standard method and could be used routinely for RNA sequencing through the Illumina^®platform.

Function:
The superior function of MCLAB's RNA-Seq Library Construction Kit depends on our proprietary enzyme systems. Unique improvement to each key enzyme at specific steps in the library construction workflow increases sensitivity, flexibility and speed for next-generation sequencing. The target RNA fragments can be tagged with linkers at both ends by RNA ligases (Cat# T4RL1-100 and T4RL2T-100). Corresponding cDNA can be synthesized by Universal Reverse Transcriptase (Cat# SSII-100). The cDNA library can be enriched by PCR with high fidelity Pfu DNA Polymerase (Cat# AD-200). After gel- or beads-based purification for size selection, final cDNA libraries can hybridize directly to the oligonucleotides on the flow cell surface for cluster generation and sequencing thereafter.

Features:
- Simplified workflow, reducing hands-on time
- Optimized reaction conditions, increasing sensitivity
- Straightforward protocol minimizing prior experience needed
- Strand-specific sequencing data
- Cost-effective with all key enzymes
- High flexibility: multiplex samples and automation friendly
- Functionally validated with Illumina^® GAIIx^TM, HiSeq^TM 2000 and MiSeq^® sequencing platforms

Recommended Storage Conditions: -20°C.