
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Read moreVisit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
Read moreWe would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
Read moreDescription:
Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant produced in E. coli is a non-glycosylated, polypeptide chain containing 511 amino acids and having a MW of 57.7 kDa. Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5'-Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.
Application:
- Synthesizes adenosine 5'-sulphatophosphate from ATP and inorganic SO42-
- Catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi)
Source:
Escherichia coli containing Yeast adenosine 5'-Triphosphate Sulfurylase gene
Unit Definition:
One unit is the amount of enzyme which incorporates 1 pmol AMP into acid-insoluble material at 37°C in 1 minute.
Unit Assay Conditions:
115 mM Tris-HCl(pH 8.0), 0.58 mM ß-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5'-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.
Recommended Storage Condition: -20ºCe.