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Human and Mouse Housekeeping Gene Primer Sets

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mHKG-100

2 mouse genes, m18S rRNA and mActß, 100rxns

Table: Validated primer sets for human and mouse housekeeping genes and their expression levels.

 

Human Genes
Mouse Genes
Name
GeneBank Accession #
Relative Expression*
Name
GeneBank Accession #
Relative Expression*
h18S rRNA
X0325
****
m18S rRNA
K01364
****
hActß
NM_001101
***
mAct²
NM_007393
***
hB2m
NM_004048
***
mB2m
NM_009735
***
hGapdh
NM_002046
***
mGapdh
NM_008084
***
hPpia
NM_021130
***
mPpia
NM_008907
***
hRpl13a
NM_012423
***
mRpl13a
NM_009438
***
hRplp0
NM_001002
**
mHsp90ab1
NM_008302
**
hHprt1
NM_000194
**
mLdhal6b
NM_175349
**
hTfrc
NM_003234
**
mHprt1
NM_013556
**
hHsp90ab1
NM_007355
*
mGusb
NM_010368
*
hGusb
NM_000181
*
mTbp
NM_013684
*
hUbc
NM_021009
*
mTfrc
NM_011638
*

 

* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.

Related Products: qHRcDNAHSMHTP

Reaction Conditions:
95°C, 10 minutes -> (95°C, 15 seconds. -> 60°C, 30 seconds.) for 40 cycles -> melting curve.

Recommended Storage Condition: -20°C


Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.

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