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gb HEMO FII (G20210A)

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The kit serves to detect G20210A mutation in coagulation factor II (Prothrombin) in human genomic DNA and is designed for clinical in vitrodiagnostics. The kit is based on real-time PCRemploying fluorescently labelled hydrolysis probes.

The kit serves to detect G20210A mutation in coagulation factor II (Prothrombin) in human genomic DNA and is designed for clinical in vitrodiagnostics. The kit is based on real-time PCRemploying fluorescently labelled hydrolysis probes.

1 pc contains reagents for 100 or 25 PCR reactions in 20 µl volume.

Product specification

  • The kit is intended for clinical in vitro diagnostics being CE IVD certified
  • Detection assay is delivered in ready-to-use format, the user merely adds the DNA sample
  • Assay working range (input DNA concentration) is 1–100 ng/µl
  • Positive controls (standards) for all three genotypes are contained in the kit, negative control is also included
  • Detection is principally based on real-time PCR employing a pair of fluorescently labelled hydrolyzation probes (allelic discrimination)
  • Detection occurs in FAM instrument channel (wild type allele) and HEX channel (mutated allele)
  • The kit has same temperature profile as other diagnostic kits from HEMO, GENETIC and PHARM group
  • The kit is validated for real-time PCR cycler types: Rotor-Gene 3000/6000/Q (Corbett Research, Qiagen), iCycler iQ5 and CFX96/CFX96Touch (Bio-Rad), ABI 7500/7500 Fast/7900HT (Applied Biosystems), AriaMx and Stratagene Mx3000P/Mx3005P (Agilent Technologies), SmartCycler (Cepheid), Mic (Bio Molecular Systems)
  • Manuals for setting respective cyclers are available for download from web pages

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