Recombinant Human FGF-3 Protein

Description
Fibroblast growth factor 3 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. 
FGF-3 has been implicated in the induction of inner ear development. Studies have suggested that FGF-3 functions synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation. During development, the activities of FGF-3 is regulated negatively by the sprouty family proteins and by Sef (similar expression to fgf genes), a transmebrane protein that shares intracellular sequence similarities with the IL-17 receptor.

Full Name
Human Fibroblast Growth Factor 3

Source
E. coli

Species
Human

Accession #
NP_005238

Molecule Weight
The mature recombinant human FGF-3 consists of 185 amino acids (28-212) and has a predicted molecular mass of 21 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblast cells. The ED50 for this effect is 0.02-0.1 µg/mL in the presence of 1 µg/mL of heparin.

 Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS