Recombinant Human FGF-8 Protein

Description
FGF-8 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. 

FGF8 supports androgen and anchorage independent growth of mammary tumor cells. FGF8 over expression increases tumor growth and angiogensis. The adult expression of FGF-8 gene is restricted to testes and ovaries. FGF8 functions as an embryonic epithelial factor. FGF8 takes part in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination.

Full Name
Fibroblast Growth Factor 8

Source
E. coli

Species
Human

Accession #
NM_006119

Molecule Weight
The mature recombinant human FGF-8 consists of 193 amino acids (23-215) and has a predicted molecular mass of 22.4 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblast cells. The ED50 for this effect is 0.25-1.5 μg/mL in the presence of 10 µg/mL heparin.

 Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS