Recombinant Human IL-12

Description
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells.  It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells.  Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. 

Full Name
Human Interleukin 12

Source
E. coli

Species
Human

Accession #
P29460 (p40) & P29459 (p35)

 Molecule Weight
Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35, aa23-219) and 40 kDa (p40, aa23-328) subunits (503 total amino acid residues).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS