Recombinant Human PDGF-AB Protein

Description
Platelet-Derived Growth Factors (PDGFs), also known as Glioma-derived growth factor (GDGF), and Osteosarcoma-derived Growth Factor (ODGF), are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF-Rα receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rβ receptor. 

Full Name
Platelet-Derived Growth Factor-AB

Source
E. coli

Species
Human

Accession #
P04085 (for chainA), Q6FHE7 (for chainB)

Molecule Weight
The mature recombinant human PDGF-AB disulfide-linked dimer is a 12.3 kDa protein containing one A chain and one B chain (234aa).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS