Recombinant Human BMP-7 Protein

Description
BMP-7 is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-7 is a potent bone inducing agent, which in the presence of appropriate osteoconductive carrier (e.g. collagen sponge or synthetic hydroxyapatite) can be used in the treatment of bone defects. The potential use of BMP-7 in dental reconstructive surgeries is currently under investigation.

Full Name
Bone morphogenetic protein 7

Source
E. coli

Species
Human

Accession #
P18075

Molecule Weight
The mature recombinant human BMP-7 consists of 139 amino acids (293-431) and has a predicted molecular mass of 15.7 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.1-0.6 µg/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS