Recombinant Human BMP-8b Protein

Description
BMP-8, also known as osteogenic protein 2 (OP2), is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-8b is required for both the initiation and maintenance of spermatogenesis. BMP-8b is also highly expressed in osteosarcomas and pancreatic cancer, and it contributes to tumor progression. BMP-8b is also expressed in adipose tissue and the hypothalamus where it contributes to the regulation of energy balance and thermogenesis.

Full Name
Bone morphogenetic protein 8b

Source
E. coli

Species
Human

Accession #
P34820

Molecule Weight
The mature recombinant human BMP-8b consists of 139 amino acids (264-402) and has a predicted molecular mass of 16 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED50 for this effect is 0.7-3.5 μg/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS