Recombinant Human BMP-9 Protein

Description
BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-9 is expressed mainly in non-parenchymal cells of the liver, but is also found in other various cells and tissues. BMP-9 can signal through the ALK1 receptor and has been implicated in a number of physiologic events. These include regulation of the hepatic reticulo-endothelial system, glucose homeostasis, and iron homeostasis, as well as the inhibition of angiogenesis. 

Full Name
Bone morphogenetic protein 9

Source
E. coli

Species
Human

Accession #
Q9UK05

Molecule Weight
The mature recombinant human BMP-9 consists of 110 amino acids (320-429) and has a predicted molecular mass of 12 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4-1.6 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS