Recombinant Human BMP-15 Protein

Description
BMP-15, also known as GDF-9b, is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-15 promotes the FSH‑independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis. It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes. BMP-15 blocks the FSH‑induced GC expression of FSH R and multiple steroidogenic molecules.

Full Name
Bone morphogenetic protein 15

Source
E. coli

Species
Human

Accession #
O95972

Molecule Weight
The mature recombinant human BMP-15 consists of 125 amino acids (268-392) and has a predicted molecular mass of 14 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS