PeqGOLD Blood RNA Kit (Safety line)

PRODUCT HIGHLIGHTS
- DNA Removing columns for efficient separation of DNA and cellular debris
- No need for organic extractions
- No need for time-consuming alcohol precipitations
- Immediate inactivation of endogenous and exogenous RNases
- Efficient separation of enzyme inhibitors and contaminants
- Final elution with RNase free water
PRINCIPLE
Erythrocytes in the sample are efficiently lysed and the leucocytes separated by centrifugation. After lysis under denaturing conditions and simultaneous inactivation of RNases the lysate is transferred to a PerfectBind column.RNA binds to the column´s silica matrix and can be washed with specific buffers to efficiently remove contaminants like hemoglobin. The RNA is finally eluted with RNase free water.
PERFORMANCE BLOOD RNA KIT
- Protocol time: < 60 min
- Column volume: 0.75 ml
- Elution volume: 50 – 100 μl
- Binding capacity: 100 μg/column
- RNA yield: 1 – 5 μg from 1 ml blood
- For up to 1 ml blood or up to 150 μl frozen blood/column
COLUMN OPTIONS
- Safety Line columns with snap-on lids
- Classic Line columns without lids 

For quick isolation of high purity total RNA from fresh or anti-coagulated treated blood, including EDTA, citrate dextrose or heparin
KIT COMPONENTS
- PerfectBind RNA columns
(Safety or Classic Line)
- Collection Tubes
- 'DNA Removing' columns
- Lysis Buffer ER
- RNA Lysis Buffer T
- RNA Wash Buffer I and II
- RNase free water
Blood RNA Kit

peqGOLD Blood RNA extraction protocol
Mix blood with Lysis Buffer ER and pellet
leucocytes by centrifugation. Wash cells with
Lysis Buffer ER and pellet by repeating the
centrifugation step. Add RNA Lysis Buffer T.
Remove debris using a 'DNA Removing'
column. Load column and centrifuge to
bind RNA to the PerfectBind matrix.
Wash once with RNA Wash Buffer I.
Wash twice with RNA Wash Buffer II.
Centrifuge to dry.
Elute RNA from the PerfectBind matrix
with RNase free water.