I-5™ Hi-Fi DNA Polymerase

General Description 
I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of from different templates including plasmids, BACS, genomic DNA, and lambda DNA. It allows for amplification of up to 8kb with human genomic DNA and up to 21kb with lambda DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows the user to save time by speeding up PCR reactions and provides higher fidelity than Taq or Pfu.

Feature 
• Robust – maximal success and minimal optimization needed
• Fidelity – 50X greater than Taq
• High Speed –10X faster than Pfu
• High Yield
• Versatile – best for long or difficult templates

Source
E. coli

Applications 
PCR
Cloning
Long or Difficult Amplification
High-Throughput PCR

Supplied with 
5X Reaction Buffer (1ml), 50mM MgCl₂ (1ml)

Supplied in (buffer description)
20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X stabilizer, pH 7.4 @ 25 C

Storage Condition 
-20°C

Unit Definition 
One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C.

References 
Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.

Shipping Condition 
dry ice

User protocol