Taq DNA Ligase

Description:
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD⁺ as a cofactor.

Figure:

Figure, Comparing to another vendor's product, the activity of MCLAB's Taq DNA ligase was tested with BstEII-digested Lamda DNA under 45°C for 15 minutes.

Application:
- Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction
- Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification

Source:
A recombinant E. coli strain carrying the cloned Taq DNA Ligase gene.

Supplied in:
10 mM Tris-HCl
50 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
0.1% TWEEN 20
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x Taq DNA Ligase Buffer

10x Taq DNA Ligase Buffer:
200 mM Tris-HCl
250 mM Potassium Acetate
100 mM Magnesium Acetate
10 mM NAD 1
100 mM DTT
0.1% Triton® X-100
pH 7.6 @ 25°C
 

Unit Definition:

One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.

Specific Activity: 400 Unit/µg

Recommended Storage Condition: -20°C