miRNA cDNA Synthesis and qPCR Kit

Description:
Small, non-coding miRNAs are widely present in eukaryotes. Many studies evidenced that miRNAs control many important physiological processes in cell development and differentiation. Therefore, quantitative assaying of miRNA is important in both basic and applied research.
miRNA cDNA Synthesis Kit provides a universal first-strand cDNA synthesis system which combines optimized polyadenylation with reverse transcription reactions based on the proprietary high quality enzymes. The robust kit is ideal for most reliable first strand synthesis with higher cDNA yields, sensitivity, and accurate quantitation from 10 pg to 1 µg of total RNA input.
The universal first-strand cDNA synthesis allows the detection of mRNA species, including β-Actin and GapDH, from the same cDNA sample. The miRNA-specific amplification could be done with target-specific PCR forward primer during the subsequent PCR reaction. When compared to traditional hybridization-based detection methods, such as Northern blot analysis, the qRT-PCR method is faster, more specific, more sensitive and using less sample material.

Features:
- Efficient reverse transcription of miRNAs into cDNA in a single step.
- Precise quantitative and accurate measurement of miRNA expression profiles with Universal qPCR Primer included in the kit gives you the flexibility to order your qPCR detection reagents separately.
- Differentiation between mature and precursor miRNA.
- Profiling of small RNAs, miRNAs, or mRNAs from a single cDNA synthesis reaction.

Recommended Storage Condition: -20°C