All-In-One 5X Reverse Transcription Mix Kit

Description:
MCLAB’s  All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR. MCLAB’s QuantumScript™ HD Reverse Transcriptase is an engineered mutation with increased sensitivity, improved specificity, maximum thermostability and reduced RNase H activity. The optimal fist-strand cDNA synthesis temperature for this enzyme is 50°C. At higher temperatures the reverse transcription reaction improves cDNA yields for difficult RNA transcription.

This Pre-mixed 5X concentrated solution includes all necessary components for the first-strand synthesis: 5X buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, QuantumScript™ HD Reverse Transcriptase, It will simplify your first strand cDNA synthesis process.

The enzyme is supplied at a concentration of 4 ul/rxn.

Features:

• Easy-to-use pre-mixed package
• High fidelity, yield and sensitivity
• Broader linear range
• Complete cDNA synthesis guaranteed
• Maximum sample volume available
• Robust and high throughput
• Works with difficult sequences

Application:
RNA reverse transcription

Recommended Storage Condition:
-20 °C

Size:

25 rxn,100 ul (cat. no. 5AIO-100)

100 rxn,400 ul (cat. no. 5AIO-200)