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https://www.carolinabiosystems.cz/1326-thickbox_default/first-strand-cdna-synthesis-kit.jpg View full size

All-In-One 5X Reverse Transcription Mix Kit

Kontakt z nami

FSCS-100

synthesis at higher temperatures

reduced RNase H activity

Descriptions:

First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB’s QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.

Applications:

  • cDNA Synthesis
  • RT-PCR
  • RT-qPCR

Recommended Storage Condition:
-20 °C

It is recommended to reduce freeze-thaw cycles as few as possible.

Components:

5X RT Buffer

RT Enzyme Mix

DTT

dNTP

Oligo dT Primer

Random Primer

Size:

50 reactions (cat. no. FSCF-100)

250 reactions (cat. no. FSCS-200)

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