PstI

Recognition site: CTGCA^G

Source:
Purified from a strain of E. coli that bears the cloned Pst I gene.

Storage Buffer:
10 mM Tris-HCl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) Triton X-100.

10x Pst I Buffer
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 μg/ml BSA
pH 7.9 @ 25°C

Reaction Condition:
Buffer at 37°C.

Typical Ligation/Recut Assay Results:
Pst I-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Pst I.

Recommended Storage Condition: -20°C.

Comments:
Does not cleave DNA when either the A or the 5’ C residue is N6 –methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 minutes, 65°C). Glycerol concentrations >5% (v/v) may alter specificity.