T7 RNA Polymerase

Description:
T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5' -> 3' synthesis of RNA starting at a T7 promoter sequence^(1,2).

Specific Activity: 312,500 U/mg

Application:
- Radiolabeled RNA probe preparation
- RNA generation for in vitro translation
- RNA generation for studies of RNA structure, processing and catalysis
- Expression control via anti-sense RNA

Source:
Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Supplied in:
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
1 mM EDTA
50% Glycerol
0.1% Triton X-100
pH 7.9 @ 25°C

Supplied with:
10x T7 RNA Polymerase Buffer
400 mM Tris-HCl
60 mM MgCl₂
100 mM DTT
20 mM Spermidine
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Recommended Storage Condition: -20°C

References:
1. Chamberlin, M, et al. (1973) J. Biol. Chem. 248, 2235-2244, 2245-2250.
2. Chamberlin, M. et al. (1982) in The Enzymes, 3rd edition, ed. P. D. Boyer (Academic Press, New York.) 15, 87-108.