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- Analiza kwasów nukleinowych
- Analiza białek
- Odczynniki biochemiczne
- Enzymy
- Edytowanie genów
- Klonowanie
- Diagnostyka kliniczna
- Human Identification STR kits
- Sprzęt laboratoryjny
- Oprogramowanie
- A&A Biotechnology
Aktualności
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description
TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreactions, to remove unbound immunologicals, and eventually for incubation solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
Applications
- Saturates binding sites on surfaces (i.e. to coat polystyrene microplates, generally combined to proteins such as BSA).
- Stabilize proteins purified in protein derivative (PPD) solution used in skin testing for tuberculosis exposure
- As a solubilizing agent of membrane proteins
- Lysing mammalian cells, at a concentration of 0.05% to 0.5% v/v, generally combined to other detergents, salts and additives.
Recommended Storage Condition: room temperature
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