Recombinant Human IL-10

Description
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Full Name
Human Interleukin 10

Source
E. coli

Species
Human

Accession #
P13232

Molecule Weight
The mature recombinant human IL-10 consists of 152 amino acids (19-178) and has a predicted molecular mass of 18.6 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. The ED50 for this effect is 0.15-0.75 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS