Recombinant Human BMP-10 Protein

Description
BMP-10 is a member of the Bone Morphogenetic Protein (BMPs) family, which is important for the development of bone and cartilage. BMPs belong to the TGF-β superfamily of structurally related signaling proteins. BMP proteins are synthesized as large precursor molecules that are cleaved by proteolytic enzymes. The active form of BMPs can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. BMPs signal through type I and type II receptor tyrosine kinases and signal through SMAD proteins.
BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation. It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.

Full Name
Bone morphogenetic protein 10

Source
E. coli

Species
Human

Accession #
O95393

Molecule Weight
The mature recombinant human BMP-10 consists of 108 amino acids (317-424) and has a predicted molecular mass of 12.2 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 15-60 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS