Nucleocapsid Protein (N-protein)

Description
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. The N-protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. The N-protein is a most abundant protein of coronavirus. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. The array of diverse functional activities accommodated in the N protein goes far beyond to be a static structural protein and makes it an interesting target in the development of antiviral therapeutics.

Source
E. coli

Organism
COVID-19

Accession #
QHO62115.1

Molecule Weight
The recombinant COVID-19 Nucleocapsid Protein consists of 420 amino acids (Met 1-Ala 419) and predicts a molecular mass of 47 kDa.

Endotoxin
< 1.0 EU per μg protein as determined by the LAL method

Purity
> 95 % as determined by SDS-PAGE

Storage
-80 °C, avoid repeated freeze-thaw cycles.