Sau3AI

Source: Recombinant. Purified from an E.coli strain carrying the cloned Sau3AI gene from Staphylococcus aureus 3A.

5′-G A T C-3′
3′-C T A G–5′

Reaction Temperature: 37°C

Prototype: MboI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x Sau3AI Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hr in a total reaction volume of 50 µl.

Note: Cleaves Dam methylated DNA in spite of sequence overlap.

Storage Conditions: Store at –20oC.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.