Mutation Surveyor

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Mutation Surveyor® software is a powerful and accurate DNA Sequencing analysis tool for Sanger Sequencing files generated by Applied Biosystems Genetic Analyzers, MegaBACE, and Beckman CEQ electrophoresis systems. Capable of performing variant analysis of up to 2000 Sanger sequencing files (.ab1, .RSD, .ESD & .scf) in 15 minutes, Mutation Surveyor delivers excellent accuracy, sensitivity, and low false positive and false negative rates in the analysis of DNA variants, including: single nucleotide polymorphisms (SNPs), insertions and deletions (INDELS), and somatic mutations in direct sequencing, PCR sequencing, Mitochondrial DNA sequencing, and resequencing projects. The package includes patented anti-correlation technology, which physically compares sample sequence traces to a reference trace, providing accuracy up to 99.5% with Phred 20 bi-directional sequences and a sensitivity of 5% of the primary peak. Along with increased accuracy and sensitivity, Mutation Surveyor features many tools for researchers and clinicians including automated download of annotated GenBank reference files, detection and deconvolution of heterozygous indels, DNA methylation detection, mitochondrial DNA sequence analysis, allele quantification, and various custom reporting options.

Mutation Surveyor software version 5.0 includes new features designed to enhance and optimize the process of variant discovery in Sanger sequencing traces. Included in version 5.0 is a new variant knowledge database, which allows users to track variants by chromosomal position, indicate false-positives and other artifacts, and query mutation database information from popular variant databases such as dbSNP, dbNSFP, and COSMIC.

Also included in Mutation Surveyor software version 5.0 is a user management system, which enables administrators to set up password protected user names, control access rights, and generate a user audit trail for each analysis project. Access rights for each user are determined by the administrator, providing assurance that unauthorized individuals do not accidentally alter a project or change the analysis parameters established by the laboratory. User management also provides user ID and Organization in the header of the final reports. Each change to the project is captured by user and time stamped.