Topomize Amplicon Library Prep Kit

Description:

MCLAB’s Topomize Amplicon Library Prep Kit is an innovative, fast and high quality Amplicon library construction kit for next generation sequencing. Targeted sequencing of large cohorts of samples is a powerful tool for the discovery and detection of disease-causing variants associated with many inherited diseases and cancers. After PCR amplification of the targeted DNA regions to generate amplicon pools, MCLAB’s Topomize Amplicon Library Prep Kit can be used to quickly add adapters with barcodes for deep sequencing.  Instead of using traditional ligase-based methods, our kit uses topoisomerase-based technology to attach adapters with barcodes onto the amplicons. The Topomize Amplicon Library Kit has unparalleled efficiency and yield with no adapter dimers or chimeras. The kit is designed for 10ng to 1µg of each pooled amplicon DNA input, and is compatible with the Illumina platforms. The fast workflow allows the amplicon library to be ready in less than 40 minutes.
 

Highlights:

• Easy and cost saving primer design: no linkers or extra nucleotides are needed except the target region primers
• More specific amplifications: primers are 100% matched to the target regions
• Versatility: adapters with barcode can be attached to any blunt end PCR products
• Unbeatable efficiency: Topoisomerase based enzymology generates higher adapter attachment rate with no bias
• High quality: no adapter dimer or other chimeras
• Fast workflow: get your amplicon library ready in less than 40 min
• All-in-One kit: includes all the reagents for sample prep and compatible with Illumina platforms

Figure:

                                                                 Topomize Amplicon Library Prep Workflow