RNase H, E. coli

Description:
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.

Specific Activity: 625,000 U/mg

Application:
- Removal of poly(A) tails of mRNA hybridized to poly(dT)
- Removal of mRNA during second strand cDNA synthesis

Source:
A recombinant E. coli strain carrying the RNAse H gene (rnh) from E. coli.

Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl₂
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C

Supplied with:
10x RNAse H Buffer

10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl₂
100 mM DTT
pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an ³H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C