View full size
Zapraszamy do odwiedzenia nas podczas jubileuszowej 20. edycji konferencji naukowej RANK 2026, która odbędzie się w dniach 18–19 marca w Pardubicach, w klubie ABC. Konferencja jest organizowana przez ...
Czytaj więcejWe are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
Czytaj więcejIn the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Czytaj więcej
View full size
Description:
PcrA is an ATP-dependent 3′ to 5′ helicase responsible for unwinding double-stranded DNA (dsDNA). PcrA binds to dsDNA and separates it into two single-stranded DNA (ssDNA) molecules.
The enzyme is supplied at 100 μg, 0.5 mg/ml.
10X Reaction Buffer (500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9)
Applications:
Helicase-dependent DNA isothermal amplification.
Experiment Result:
Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.

Source:
Expressed in E. coli
Storage:
-20°C