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- Analiza kwasów nukleinowych
- Analiza białek
- Odczynniki biochemiczne
- Enzymy
- Edytowanie genów
- Klonowanie
- Diagnostyka kliniczna
- Human Identification STR kits
- Sprzęt laboratoryjny
- Oprogramowanie
- A&A Biotechnology
Aktualności
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description:
PcrA is an ATP-dependent 3′ to 5′ helicase responsible for unwinding double-stranded DNA (dsDNA). PcrA binds to dsDNA and separates it into two single-stranded DNA (ssDNA) molecules.
The enzyme is supplied at 100 μg, 0.5 mg/ml.
10X Reaction Buffer (500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9)
Applications:
Helicase-dependent DNA isothermal amplification.
Experiment Result:
Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.
Source:
Expressed in E. coli
Storage:
-20°C
Koszyk
produkt
(pusty)
