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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
- AdvancedSeq
- BioDynami
- Plant Cell Technology
News
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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Description:
PcrA is an ATP-dependent 3′ to 5′ helicase responsible for unwinding double-stranded DNA (dsDNA). PcrA binds to dsDNA and separates it into two single-stranded DNA (ssDNA) molecules.
The enzyme is supplied at 100 μg, 0.5 mg/ml.
10X Reaction Buffer (500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9)
Applications:
Helicase-dependent DNA isothermal amplification.
Experiment Result:
Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.
Source:
Expressed in E. coli
Storage:
-20°C
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