MC Cloning 5’/3’-RACE Kit

Description
The MC Cloning 5'/3' RACE Kit provides a convenient method for performing both 5' and 3' rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest. MCLAB's SmartRT reverse transcriptase is an engineered MMLV RT with increased thermostability, improved cDNA synthesis ability, and a reduced RNase H activity. The enzyme also has a terminal transferase activity. This tailing activity of the SmartRT reverse transcriptase allows to synthesize complete cDNA by SMART (Switching Mechanism At 5' end of RNA Transcript) cDNA synthesis technology. With a few additional nucleotides added to the 3' end of the first-strand cDNA, 5' SMART universal oligo, containing terminal complementation to the nucleotides at 3' end of the first-strand cDNA, can be annealed to first-strand cDNA tail and serves as an extended template for RT. The switch of template from mRNA to 5' SMART universal oligo produces a complete cDNA copy of transcript RNA with 5' SMART universal oligo at the end. The cDNA transcript from SmartRT can be used directly in 5' and 3' RACE with MCLAB's I-5™ 2X High-Fidelity Master Mix.

Features
Specific full length cDNA enrichment technique
Optimized primer set for full length cDNA sequencing
Simplified protocol without 5' end adaptor-ligation step

Applications
Generating full-length cDNAs
Structural and expression studies of RNA molecules
Isolation and characterization of 5′ or 3′ ends from low-copy RNA messages
Amplification and further cloning of rare mRNAs
Use in conjunction with exon-trapping methods

Components
1.

Storage Condition: -20 °C

2.

5' SMART Universal Oligo, 10 µl/20 µl.

Storage Condition: -80 °C