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    In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...

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First Strand cDNA Synthesis Kit

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FSCS-100

First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.

Description:
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB's QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.

 

Applications:

cDNA Synthesis

RT-PCR

RT-qPCR

 

Components:

Components

Cap Color

Volume (50 rxn)

Volume (250 rxn)

5X RT Buffer

Red

125 μl

625 μl

RT Enzyme Mix

Clear

100 μl

500 μl

DTT

Yellow

50 μl

250 μl

dNTP

Blue

50 μl

250 μl

Oligo dT Primer

Green

50 μl

250 μl

Random Primer

Purple

50 μl

250 μl

 

Storage Condition:
-20 °C. It is recommended to reduce freeze-thaw cycles as few as possible.

 

Shipping Condition: Dry ice

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