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We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
Czytaj więcejIn the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Czytaj więcejVisit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Description:
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB's QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.
Applications:
cDNA Synthesis
RT-PCR
RT-qPCR
Components:
|
Components |
Cap Color |
Volume (50 rxn) |
Volume (250 rxn) |
|
5X RT Buffer |
Red |
125 μl |
625 μl |
|
RT Enzyme Mix |
Clear |
100 μl |
500 μl |
|
DTT |
Yellow |
50 μl |
250 μl |
|
dNTP |
Blue |
50 μl |
250 μl |
|
Oligo dT Primer |
Green |
50 μl |
250 μl |
|
Random Primer |
Purple |
50 μl |
250 μl |
Storage Condition:
-20 °C. It is recommended to reduce freeze-thaw cycles as few as possible.
Shipping Condition: Dry ice