First Strand cDNA Synthesis Kit

Reference: FSCS-100

First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.

More info

Description:
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB's QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.

Applications:

cDNA Synthesis

RT-PCR

RT-qPCR

Components:

Components	Cap Color	Volume (50 rxn)	Volume (250 rxn)
5X RT Buffer	Red	125 μl	625 μl
RT Enzyme Mix	Clear	100 μl	500 μl
DTT	Yellow	50 μl	250 μl
dNTP	Blue	50 μl	250 μl
Oligo dT Primer	Green	50 μl	250 μl
Random Primer	Purple	50 μl	250 μl