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1M IPTG

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IPTG-100

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.

Description
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon (1).

Applications

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1)

Induction of gene expression under control of the lac promoter

 

Empirical Formula: C9H18O5S

Recommended Storage Condition: -20oC. We recommend making single-use aliquots to prevent repeated freeze-thaw cycles of IPTG in solution.

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