Description
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon (1).
Applications
Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1)
Induction of gene expression under control of the lac promoter
Empirical Formula: C9H18O5S
Recommended Storage Condition: -20oC. We recommend making single-use aliquots to prevent repeated freeze-thaw cycles of IPTG in solution.