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RANK 2026
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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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Description:
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli. The fusion protein, which contains a GST tail can then be purified through affinity chromatography. MCLAB’s glutathione agarose is designed for the specific purification of GST recombinant proteins and other glutathione-binding proteins. Glutathione agarose is uniquely formulated for excellent binding capacity and purity of the protein of interest.
Specifications
| Chromatography technique | GST-tagged protein purification |
| Matrix | Highly cross-linked 6% beaded agarose |
| Active group | Glutathione |
| Active group density | >40 umol/ml drained medium |
| Binding Capacity | > 25 mg horse liver GST/ml resin |
| Spacer | 1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
| Bead geometry & size | Spherical 50 to 150 um |
| Bead mean diameter d50v | 90 um |
| pH Stability working range | 4 to 13 |
| pH Stability cleaning in place (cip) | 4 to 13 |
| Chemical stability | Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
| Storage | 2 to 8 °C in 20% Ethanol |
Recommended Storage Conditions: 2ºC to 8ºC.
Binding Buffer:
50 mM Tris
150 mM NaCl
pH 8.0
Elution Buffer:
50 mM Tris pH 7.8
0.15 M NaCl
1 mM EDTA
1 mM DTT
10 mM GST
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