Glutathione Agarose Beads

Reference: GAB-100

Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.

More info

Description:
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli. The fusion protein, which contains a GST tail can then be purified through affinity chromatography. MCLAB’s glutathione agarose is designed for the specific purification of GST recombinant proteins and other glutathione-binding proteins. Glutathione agarose is uniquely formulated for excellent binding capacity and purity of the protein of interest.

Specifications

Chromatography technique	GST-tagged protein purification
Matrix	Highly cross-linked 6% beaded agarose
Active group	Glutathione
Active group density	>40 umol/ml drained medium
Binding Capacity	> 25 mg horse liver GST/ml resin
Spacer	1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage)
Bead geometry & size	Spherical 50 to 150 um
Bead mean diameter d50v	90 um
pH Stability working range	4 to 13
pH Stability cleaning in place (cip)	4 to 13
Chemical stability	Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
Storage	2 to 8 °C in 20% Ethanol

Recommended Storage Conditions: 2ºC to 8ºC.

Binding Buffer:
50 mM Tris
150 mM NaCl
pH 8.0

Elution Buffer:
50 mM Tris pH 7.8
0.15 M NaCl
1 mM EDTA
1 mM DTT
10 mM GST