Aktualności

1st Czechoslovak Congress of Medical Genetics 2025

In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Czytaj więcej
RANK 2025

Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
Czytaj więcej
Kapras Day 2025

We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
Czytaj więcej

Archiwum aktualności

https://www.carolinabiosystems.cz/814-thickbox_default/set-primeru-pro-analyzu-housekeeping-genu-cloveka-a-mysi.jpg

View full size

Human and Mouse Housekeeping Gene Primer Sets

Kontakt z nami

Indeks: hHKG-100 a mHKG-100

Validated primer sets for human and mouse housekeeping genes and their expression levels.

Więcej informacji

able: Validated primer sets for human and mouse housekeeping genes and their expression levels.

Human Genes			Mouse Genes
Name	GeneBank Accession #	Relative Expression*	Name	GeneBank Accession #	Relative Expression*
h18S rRNA	X0325	****	m18S rRNA	K01364	****
hActß	NM_001101	***	mAct²	NM_007393	***
hB2m	NM_004048	***	mB2m	NM_009735	***
hGapdh	NM_002046	***	mGapdh	NM_008084	***
hPpia	NM_021130	***	mPpia	NM_008907	***
hRpl13a	NM_012423	***	mRpl13a	NM_009438	***
hRplp0	NM_001002	**	mHsp90ab1	NM_008302	**
hHprt1	NM_000194	**	mLdhal6b	NM_175349	**
hTfrc	NM_003234	**	mHprt1	NM_013556	**
hHsp90ab1	NM_007355	*	mGusb	NM_010368	*
hGusb	NM_000181	*	mTbp	NM_013684	*
hUbc	NM_021009	*	mTfrc	NM_011638	*

* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.

Reaction Conditions:
95 °C, 10 minutes -> (95 °C, 15 seconds. -> 60 °C, 30 seconds.) for 40 cycles -> melting curve.

Recommended Storage Condition: -20 °C

Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.