Human and Mouse Housekeeping Gene Primer Sets

Reference: hHKG-100 a mHKG-100

Validated primer sets for human and mouse housekeeping genes and their expression levels.

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Table: Validated primer sets for human and mouse housekeeping genes and their expression levels.

Human Genes			Mouse Genes
Name	GeneBank Accession #	Relative Expression*	Name	GeneBank Accession #	Relative Expression*
h18S rRNA	X0325	****	m18S rRNA	K01364	****
hActß	NM_001101	***	mAct²	NM_007393	***
hB2m	NM_004048	***	mB2m	NM_009735	***
hGapdh	NM_002046	***	mGapdh	NM_008084	***
hPpia	NM_021130	***	mPpia	NM_008907	***
hRpl13a	NM_012423	***	mRpl13a	NM_009438	***
hRplp0	NM_001002	**	mHsp90ab1	NM_008302	**
hHprt1	NM_000194	**	mLdhal6b	NM_175349	**
hTfrc	NM_003234	**	mHprt1	NM_013556	**
hHsp90ab1	NM_007355	*	mGusb	NM_010368	*
hGusb	NM_000181	*	mTbp	NM_013684	*
hUbc	NM_021009	*	mTfrc	NM_011638	*

* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.

Reaction Conditions:
95 °C, 10 minutes -> (95 °C, 15 seconds. -> 60 °C, 30 seconds.) for 40 cycles -> melting curve.

Recommended Storage Condition: -20 °C

Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.