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Table: Validated primer sets for human and mouse housekeeping genes and their expression levels.
|
Human Genes |
Mouse Genes |
||||
|
Name |
GeneBank Accession # |
Relative Expression* |
Name |
GeneBank Accession # |
Relative Expression* |
|
h18S rRNA |
X0325 |
**** |
m18S rRNA |
K01364 |
**** |
|
hActß |
NM_001101 |
*** |
mAct² |
NM_007393 |
*** |
|
hB2m |
NM_004048 |
*** |
mB2m |
NM_009735 |
*** |
|
hGapdh |
NM_002046 |
*** |
mGapdh |
NM_008084 |
*** |
|
hPpia |
NM_021130 |
*** |
mPpia |
NM_008907 |
*** |
|
hRpl13a |
NM_012423 |
*** |
mRpl13a |
NM_009438 |
*** |
|
hRplp0 |
NM_001002 |
** |
mHsp90ab1 |
NM_008302 |
** |
|
hHprt1 |
NM_000194 |
** |
mLdhal6b |
NM_175349 |
** |
|
hTfrc |
NM_003234 |
** |
mHprt1 |
NM_013556 |
** |
|
hHsp90ab1 |
NM_007355 |
* |
mGusb |
NM_010368 |
* |
|
hGusb |
NM_000181 |
* |
mTbp |
NM_013684 |
* |
|
hUbc |
NM_021009 |
* |
mTfrc |
NM_011638 |
* |
* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.
Reaction Conditions:
95 °C, 10 minutes -> (95 °C, 15 seconds. -> 60 °C, 30 seconds.) for 40 cycles -> melting curve.
Recommended Storage Condition: -20 °C
Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.
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