Alkalická fosfatáza SAP1

Description:
Alkaline Phosphatase SAP1 catalyzes the hydrolysis of phosphate monoesters. Has higher turnover rate and milder conditions of inactivation than bacterial enzyme. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Used to remove 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. Applicable to dephosphorylation of proteins.

Storage Buffer:

10 mM Tris-HCl (pH 8.0 at 22°C)

50 mM KCl

1 mM MgCl2

0.1 mM ZnCl2

50% (v/v) glycerol

Unit Definition:
One unit is the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenylphosphate to p-nitrophenol in 1 min at 37oC in a buffer of 1 M diethanolamine, 10 mM p-nitrophenylophosphate, 0.25 mM MgCl2 (pH 9.8).

Recommended Storage Condition: -20°C