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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
- AdvancedSeq
- BioDynami
- Plant Cell Technology
News
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RANK 2026
Visit us at the jubilee 20th edition of the RANK 2026 professional conference, which will take place on March 18–19 in Pardubice at the ABC Club. The conference is organized by the Czech Society of Cl...
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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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Description:
Alkaline Phosphatase SAP1 catalyzes the hydrolysis of phosphate monoesters. Has higher turnover rate and milder conditions of inactivation than bacterial enzyme. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Used to remove 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. Applicable to dephosphorylation of proteins.
10x Alcalic fosfatase I buffer (1,5 ml)
Source:
Pandalus borealis
Storage buffer:
25 mM Tris-HCl
1 mM MgCl2
50% (v/v) glycerol.
pH 7.6, 4 °C
Unit Definition:
One unit is the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenylphosphate to p-nitrophenol in 1 min at 37oC in a buffer of 1 M diethanolamine, 10 mM p-nitrophenylophosphate, 0.25 mM MgCl2 (pH 9.8).
Recommended Storage Condition: -20°C
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