Product Description
MCLAB's All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize first strand cDNA for RT-PCR and RT-qPCR. MCLAB's QuantumScript™ HD Reverse Transcriptase is an engineered mutation with increased sensitivity, improved specificity, maximum thermostability and reduced RNase H activity. The optimal fist-strand cDNA synthesis temperature for this enzyme is 50°C. The higher temperatures reverse transcription reaction improves cDNA yields for difficult RNA transcription. This Pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis: 5X buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, QuantumScript™ HD Reverse Transcriptase, It will simplify your first strand cDNA synthesis process.
Features
Easy-to-use pre-mixed package
High fidelity, yield and sensitivity
Broader linear range
Complete cDNA synthesis guaranteed
Maximum sample volume available
Robust and high throughput
Work with difficult sequences
Applications
RNA reverse transcription
Recommended Storage Condition
-20 °C
Shipping Condition
dry ice
The Ab cloning RACE kit provides a novel method for performing both 5' and 3’ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning both heavy and light chains of human and mouse antibodies.
Choo-Choo CloningTM Kits are highly efficient directional PCR cloning kits for rapid, ligase and restriction enzyme-independent PCR cloning.
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.
The MC Cloning 5'/3' RACE Kit provides a convenient method for performing both 5' and 3' rapid amplifications of cDNA ends (RACE) with optimized primers for cloning any gene of interest.
25 rxn(4ul/rxn),100 μl
Čeština
English
