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Description:
Magnetic beads for genomic DNA (gDNA) purification from the American company BioDynami combine BioDynami's patented chemistry with the unique properties of SPRI beads (Solid Phase Reversible Immobilization). They effectively eliminate contaminating RNA and recover gDNA in a single reaction step. Additionally, unwanted components such as proteins, dNTPs, salts, enzymes, and other impurities can also be removed in the same step. The genomic DNA is then ready for downstream applications such as PCR, qPCR, sequencing, and enzymatic processing, etc.
Workflow:
In the first step (gDNA Binding RNA depletion), genomic DNA binds to the magnetic beads while RNA is simultaneously removed. This is followed by magnetic separation (Magnetic Separation), where the beads with bound DNA are captured using a magnet, and unwanted contaminants are removed. In the washing step (Washing), the beads are washed to remove any remaining impurities. Finally, in the elution step (Elution), the purified genomic DNA is released from the beads into the elution buffer.
Efficiency:
The graph on the left shows the yield of genomic DNA before (Input) and after (Purified) purification, with the magnetic bead purification maintaining a high DNA recovery rate. The electrophoretic gel on the right demonstrates the presence (Purification +) and absence (Purification -) of RNA contamination in the DNA sample after purification. It shows that after purification with magnetic beads, RNA is effectively removed, resulting in clean genomic DNA suitable for downstream applications.
Features:
- Effective removal of RNA contamination by RNase
- Removal of unwanted components and impurities
- High recovery rate of genomic DNA
Package size:
40 reactions (Cat. No. 40060S)
200 reactions (Cat. No. 40060L)
40060S
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