Description
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus. It consists of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many of mammalian species, most notably IgGs. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE. It binds the heavy chain with the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupt opsonization and phagocytosisties.
Applications
Antibody purification
Protein purification
Isolates immune complexes
Source
Purified from E.coli that carries the protein A gene of Staphylococcus aureus.
Purity
>98% by SDS-PAGE and HPLC analyses
Recommended Storage Condition: 4 °C
Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
Used in proteomics and protein chromatography, amino acid agarose is prepared by covalently coupling of amino acid through a 17 atom stable and uncharged ether hydrophilic linkage to highly cross-linked 6% agarose with bead mean diameter of 90 um.
Ni2+, Co2+, Cu2+, or Zn2+ charged nitrilotriacetic acid (NTA) coupled to Agarose CL-6B via a stable and uncharged long ether hydrophilic spacer arm, and offers high binding capacity and minimal non-specific binding.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
Q Cross-linked Agarose High Performance has well-deserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules.
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensure maintained speed even with viscous samples.
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Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus.
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