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Source: Anabaena variabilis
5′-G G W C C-3′
3’-C C W G G-5’
Reaction Temperature: 37°C
Prototype: AvaII
Inactivation Temperature (20 min): 65°C
Reaction Buffer:
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.
RE20071
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