NotI (reduced star activity)

NotI (reduced star activity)

RE22965 - 500 U

RE229625 - 2 500 U

Liczba: 500 U

Source: Nocardia otitidis-caviarum

5’-G C G G C C G C-3’
3’-C G C C G G C G-5’

Purified from an E.coli strain carrying the cloned and purified NotI gene from Nocardia otitidis-caviarum.

Reaction Temperature: 37°C

Prototype: NotI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, for nickase as well as for nonspecific single- and double-stranded DNase activities. Ligation / recut assays passed successfully.

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Endonuclease VIII, E. coli

Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site).