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Source: Thermus thermophilus 111
5’-G A C N N N G T C-3’
3’-C T G N N N C A G-5’
Reaction Temperature: 65°C
Prototype: Tth111I
Inactivation Temperature (20 min): —
Reaction Buffer:
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.
RE24201
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