I-5™ Hi-Fi HotStart DNA Polymerase

General Description
I-5 Hi-Fi DNA HotStart Polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of difficult templates including plasmids, BACS, genomic DNA, and lambda DNA. It allows the amplification of up to 8kb human genomic DNA and up to 21kb lambda DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows users to save time by speeding up PCR reactions and provides higher fidelity than Taq or Pfu. The enzyme contains a HotStart mechanism that inactivates the enzyme until it is heated. This allows users to setup PCR reactions at room temperature without worrying about primer dimers or non-specific preamplification.

Feature
• Fast – 4X faster than hot start Taq
• Robust – high inhibitor tolerance
• High yields – high efficiency
• Long PCR products

Source 
E. coli

Applications 
Hot Start PCR
Routine PCR
Fast PCR
High throughput PCR
Genotyping

Supplied with 
5X Reaction Buffer (1ml), 50mM MgCl₂ (1ml)

Supplied in (buffer description) 
20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X stabilizer, pH 8.0 @ 25 C

Storage Condition 
-20ºC

Unit Definition 
One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C.

Reference 
Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.

Shipping Condition
dry ice

User protocol