Uracil DNA Glycosylase (UDG)

Description:
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

Application:
- Control of carry-over contamination in PCR ⁽¹⁾
- Glycosylase mediated single nucleotide polymorphism detection (GMPD) ⁽²⁾
- Site-directed mutagenesis ⁽³⁾
- As a probe for protein-DNA interaction studies ⁽⁴⁾
- SNP genotyping
- Cloning of PCR products ⁽⁵⁾
- Generation of single strand overhangs of PCR products and cDNA

Source:
A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.

Specific Activity: 77,000 U/mg

Supplied With:
10X UDG Reaction Buffer
Incubate at 37°C

10X UDG Reaction Buffer:
200mM Tris-HCl
10 mM DTT
10 mM EDTA
pH 8.0 @ 25°C

Supplied In:
10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.

Recommended Storage Condition: -20°C