LbCas12a (D832A, with an Asp832→Ala substitution)
Origin: Lachnospiraceae bacterium (strain ND2006)
Purity > 95%
Description:
Cas12a (also referred as Cpf1) nuclease, belongs to the Class 2, Type V CRISPR system. dCas12a from Lachnospiraceae bacterium (strain ND2006) is a catalytically inactive LbCas12a (D832A, with an Asp832→Ala substitution). The protein can be programmed with a gRNA and directed to bind at a desired sequence of DNA.
The enzyme's purity is greater than 95%.
Application:
Source:
dCas12a( D832A) from Lachnospiraceae bacterium (strain ND2006) expressed in E. coli.
Storage:
-20°C
Size:
200 pmol, 200 µl (1 µM) (cat.n. dCAS12A-100)
10X Cas12a Reaction Buffer 200 µl (cat.n. CAS12A-BUF-100-D)
10X Cas12a Reaction Buffer 1 ml (cat.n. DCAS12A-BUF-200)
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.
Benzonase endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity.
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
CRISPR-Cas systems are powerful tools for mediating nucleic acids. Cas12a (also referred as Cpf1) nuclease belonging to the Class 2, Type V CRISPR system.
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
origin: E. coli carrying the Tn5 gene
random transposon insertion into DNA
requires 19-bp ME sequence
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
LbCas12a (D832A, with an Asp832→Ala substitution)
Origin: Lachnospiraceae bacterium (strain ND2006)
Purity > 95%
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